Determination of polyphenols in Chinese jujube using ultra-performance liquid chromatography–mass spectrometry

Abstract The polyphenolic composition of the same variety winter jujube was determined using ultra-performance liquid chromatography–mass spectrometry (UPLC–MS/MS). A metabolomic approach employed to determine polyphenols at different developmental stages (S1, S2, and S3). total phenolic content Chinese quantified, metabolites were statistically analyzed orthogonal partial least squares discriminant analysis (OPLS-DA) for differential metabolite screening clustering key components. findings revealed that 128 components had been identified. Different periods could not be clearly distinguished in principal component analysis, there a crossover between S2 S3 stages. In contrast, OPLS-DA score plot effectively distinguish samples periods, its visualized by volcano based on OPLS-DA. Ten clustered analyzed, among which N ′- p -coumaroylguanidinium, - -coumaroylbutylamine, caffeoyl guanidinium, -feruloyl pianoside, isorhamnetin 5- O -hexoside, -acetyl-hexoside, quercetin, lignan -hexosyl- -pentoside higher S1 period chrysoeriol 6- C -hexoside periods. this study, differences elucidated, provided scientific guidance application polyphenols.